Beatson Advanced Imaging Resource (BAIR)
Head: Leo Carlin
Beatson Advanced Imaging Resource (BAIR) scientists work closely with the Institute's researchers to uncover and interrogate important molecular pathways in cancer. The BAIR is thus involved at some stage in nearly every paper from researchers at the Beatson that contains a light micrograph. We train scientists in all stages of modern microscopical research from advice on sample preparation, basic and advanced microscope operation and data acquisition through to quantitative image analysis and interpretation. We have a wide range of specialised microscopes within the facility that provide users with the flexibility to approach their research using a diverse range of applications. For example, two colour fluorescent protein imaging can be imaged in widefield, TIRF and confocal. FLIM-FRET can be performed in widefield, TIRF, Spinning Disk or MPLSM.
At present, we are concentrating on the development of live cell in vivo imaging within the Institute. The arrival of the new Zeiss LSM 880 'Airyscan FAST' with the additional multiphoton capability will massivly increase our ability to study this. We are particularly excited about using this new microscope to study tumour cell biology and immunology of primary and metastatic lung, liver, brain and pancreatic tumours that will benefit from the increased speed and resolution of the Zeiss 880MP system.
- Priority given to Beatson users, followed by University of Glasgow
- Outside access primarily on a collaborative basis
- E-mail or approach a member of the facility staff in the first instance to discuss your microscopy needs
- Live cell imaging, including time and frequency domain FLIM, TIRF, photo-activation, photo-bleaching and photo-conversion
- Mouse in vivo imaging, including collagen SHG and use of photo-activation and photo-bleaching, FLIM and FRET
In vivo imaging