High-Content Analysis

The Screening Facility couples genetic and chemical high throughput screening with multiparametric phenotypic image analysis to support translational cancer research. We employ functional genomics (siRNA, shRNA, CRISPR) tools in both pooled and arrayed approaches to support target identification and target validation in multiple types of cancer. Our state-of-the-art image analysis capabilities, including a machine learning module, support identification of a suite of cellular phenotypes in both 2D and 3D environments.

Our Perkin Elmer Opera and Operetta High-Content Screening systems are supported by a robotic plate-loading arm, and JANUS and Wellmate liquid handling systems.

High-Content Analysis Equipment

Opera Phenix

Opera

The Opera Phenix is a high-throughput confocal microscope primarily used for high-content screening and phenotype analysis. The system supports automated plate loading and image acquisition. The Opera Phenix is particulary advanced in 3D imaging of cells, organoids, spheroids and tissues. Coupled to Harmony/Columbus analysis software, the Opera Phenix can generate large amounts of data suitable for bioinformatics and machine learning.

Opera segmentation

High-Content Analysis Team

Lynn McGarry

l.mcgarry@beatson.gla.ac.uk

High Content Screening
Screening Image Analysis
Machine Learning


mcgarry lynn

John Halpin

John.Halpin@glasgow.ac.uk

Image Analysis
Bioinformatics
Machine Learning


John pic

Research

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Read more about the Research Groups working at the Beatson Institute.

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Seminars

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Find out more about our seminars including our Distinguished Seminar Programme.

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