The facility provides a range of histology and immunohistochemistry services for the research groups. It offers routine processing for all types of fixed tissue for paraffin-embedded histology, cryomicrotomy, cell pellet processing, H+E staining and special histology stains. A Thermo Excelsior machine offers large-scale tissue processing, while a Finesse microtome allows consistent sectioning quality. Material for PCR investigation can be sectioned from both paraffin-embedded and frozen material. Immunohistochemistry and immunofluorescence work can be undertaken with the laboratory utilising a Dako Autostainer for large-scale immunohistochemistry investigations.
The service provides routine sequencing of plasmids using an Applied Biosystems 3130xl (16 capillary) sequencer with a turnaround time of 24 hours. It also has the capacity to do next generation sequencing with an Illumina GAIIx sequencer applying ChIP-seq and RNA-seq protocols. A Qiagen 8000 Robot is used for routine small and large-scale DNA purification from overnight bacterial cultures.
This service provides a diverse range of support including: