BAIR equipment

Confocal Microscopes

Confocal microscopy is a very powerful tool and has many advantages over wide-field fluorescent microscopy. The use of a pinhole enables the user to eliminate the out of focus light and permits optical sectioning of cell or tissue samples. BAIR supports a number of standard and super-resolution confocal systems, which are also coupled to advanced technologies such as FLIM/FRET, TIRF or in vivo imaging.

Zeiss LSM 880 with Airyscan

Zeiss LSM 880

The Zeiss LSM 880 with Airyscan is a super-resolution-capable confocal microscope. The 'Airyscan' detector delivers increased resolution (1.7x) with high sensitivity at around 140nm laterally and 400nm axially. The 'Airyscan Fast' mode increases scanning speeds by a factor of 4. This allows users to image at a higher speed without sacrificing sensitivity or resolution. It has a full cage environmental chamber for live cell imaging and is also suitable for photoactivation, FRAP and FCS.

Airyscan Principle: https://www.youtube.com/watch?v=QjKrLTHGnc8

Airyscan Fast: https://www.youtube.com/watch?v=ln6VUmLzLLc

Savvas 880Savvas Nikolaou

Nikki 880 Nikki R. Paul

Nikon A1R

nikon A1R

A fully automated, highly sensitive confocal microscope system, the Nikon A1R is built around a Nikon Ti Eclipse microscope with (Perfect Focus System) PFS. The system has a full cage environmental chamber and is therefore suitable for live cell timelapse imaging.

The Nikon A1R has two scanning systems: Firstly, there is a conventional sawtooth scanning system – galvano, which is used for high-resolution imaging where speed is not essential. Secondly, there is a fast resonant scanner, which is used for applications where the need for speed outweighs the need for high resolution. The two scanning systems can be operated in rapid succession or simultaneously. This system is therefore also suitable for photoactivation and FRAP experiments.

AJ NikonAmelie Juin

Olympus FV1000

FV1000

The Olympus FV1000 is a confocal system designed for high resolution confocal imaging of both fixed and living cells. The FV1000 is built around a BX81 inverted microscope and  is equipped with a SIM scanner that enables synchronisation of specimen excitation and observation (while one laser stimulates the second laser simultaneously provides high resolution imaging). This makes the FV1000 suitable for FRAP, FLIP and photoactivation experiments.

Anh OlympusAnh Hoang Le

Widefield Microscopes

The Beatson Institute is equipped with a wide range of microscopes for multiple applications. These can be used for imaging tissues, cells or even nanoparticles such as exosomes. Microscopy techniques can also be coupled and complementary to other advanced technologies such as proteomics and metabolomics.

Olympus BX51

BX51

The BX51 microscope is a widefield system available for bright field and fluorescence observation methods. It is a non-motorised upright microscope system which is used primarily for fixed histological tissue sections and cells. It is also used for fixed fluorescently labelled samples.

http://olympus.magnet.fsu.edu/primer/java/lightpaths/bx51fluorescence/index.html 

BX51 pancreas

BX51 Fluorescence

 

 

 

 

 

 

Nanosight LM10

Nanosight

The Nanosight LM10 allows rapid and accurate analysis of the size distribution and concentration of all types of nanoparticles from 10nm to 2000nm in diameter, depending on the instrument configuration and sample type. The nanosight can be used to detect exosomes and microvesicles which are secreted from cells and tissues.

Leica Laser Microdissection

LMD

Laser microdissection uses a laser to isolate specific microscopic regions from tissue samples. The system is built around  a Leica DM6000B upright microscope stand.  These samples can be further analysed using additional techniques such as proteomics and metabolomics.

Zeiss Microinjection

MI

The microinjection system consists of a Zeiss Axio Observer A1 microscope equipped with an Eppendorf Femtojet and Inject man N12. This system is mainly used to inject cells with nucleic acids or therapeutics.  

Timelapse Microscopes

BAIR supports a range of long-term live-cell imaging microscopes with both manual and automated setups and analysis software.

Phasefocus

phasefocus

The Phasefocus VL21 Live Cell Imaging System permits label-free long-term timelapse imaging of live-cells. The Phasefocus uses a quantitative phase imaging (QPI) technology known as Ptychography to detect cells. Subsequent analysis can be used to characterise cell migration, morphology and proliferation over time.

Phasefocus Example

 

 

 

 

 

 

www.phasefocus.com

Incucyte Zoom (1/3/4)

incucyte 2

The Incucyte Zoom Live-Cell Imaging systems are automated microscopes that fit within tissue culture incubators. This permits long-term analysis of 2D and 3D cultures in multiple culture plate formats. The Incucye Zoom systems can also be used to detect fluorescent proteins or dyes, which are often used to measure cell proliferation and apoptosis. In combination with the WoundMaker tool, the Incucyte Zoom systems are often used to monitor Scratch Would Cell Migration over time in a 96-well plate format.

 incucyte1

 https://www.essenbioscience.com/en/products/incucyte/

Scratch Wound Zoom

Incucyte S3

Spheroid S3

The Incucyte S3 Live-Cell Imaging system is an automated microscope that fits within a tissue culture incubator. This permits long-term analysis of 2D and 3D cultures in multiple culture plate formats. The Incucyte S3 system can also be used to detect fluorescent proteins or dyes, which are often used to measure cell proliferation and apoptosis. In combination with the WoundMaker tool, the Incucyte S3 can be used to monitor Scratch Would Cell Migration over time in a 96-well plate format.

The Incucyte S3 has additional software modules such as the Spheroid analysis module, which permits measurement of 3D tumouroids and organoids over time.

https://www.essenbioscience.com/en/products/incucyte/incucyte-s3/

Super-resolution Microscopes

Zeiss LSM 880 with Airyscan

Zeiss LSM 880

The Zeiss LSM 880 with Airyscan is a super-resolution-capable confocal microscope.   The 'Airyscan' detector delivers increased resolution (1.7x) with high sensitivity at around 140nm laterally and 400nm axially. The 'Airyscan Fast' mode increases scanning speeds by a factor of 4. This allows users to image at a higher speed without sacrificing sensitivity or resolution. It has a full cage environmental chamber for live cell imaging and is also suitable for photoactivation, FRAP and FCS.

Airyscan Principle: https://www.youtube.com/watch?v=QjKrLTHGnc8

Airyscan Fast: https://www.youtube.com/watch?v=ln6VUmLzLLc

Savvas 880Savvas Nikolaou

Nikki 880 Nikki R. Paul

Zeiss Multiphoton 880

The Zeiss Multiphoton 880 with Airyscan is a super-resolution-capable confocal microscope. This system is in an upright configuration, and is suited to in vivo imaging of e.g. skull/brain, lung sections and abdominal organs with the use of vacuum windows.

880 MP Fred Frederic Fercoq

880 MP AmandaAmanda Mcfarlane

Elyra 7

Multiphoton Microscopes

Zeiss Multiphoton 880

The Zeiss Multiphoton 880 with Airyscan is a super-resolution-capable confocal microscope. This system is in an upright configuration, and is suited to in vivo imaging of e.g. skull/brain, lung sections and abdominal organs with the use of vacuum windows.

880 MP Fred Frederic Fercoq

880 MP AmandaAmanda Mcfarlane

Trimscope 1

TRIM

The LaVision TRIM microscope is a high-resolution multiphoton system able to image small groups of cells down to the level of subcellular structure and generate three-dimensional reconstructions. It is capable of fast and sectioned FLIM, as well as detecting second-harmonic generation (SHG).  The system has a heated stage and CO2 so is suitable for live-cell imaging. 

This system has been used to image neurons of the central nervous system and epithelial cells within the crypts of the colon.

Trimscope 2

The LaVision TRIM microscope is a high-resolution multiphoton system able to image small groups of cells down to the level of subcellular structure and generate three-dimensional reconstructions. It is capable of fast and sectioned FLIM, as well as detecting second-harmonic generation (SHG).

This system is capable of in vivo imaging using e.g. abdominal or mammary windows. In addition, this system has dual channel FLIM detectors so can do simultaneous FLIM measurements of CFP and GFP-based biosensors.

High-Content Analysis

Opera Phenix

Opera

The Opera Phenix is a high-throughput confocal microscope primarily used for high-content screening and phenotype analysis. The system supports automated plate loading and image acquisition. The Opera Phenix is particulary advanced in 3D imaging of cells, organoids, spheroids and tissues. Coupled to Harmony/Columbus analysis software, the Opera Phenix can generate large amounts of data suitable for bioinformatics and machine learning.

Opera segmentation

FLIM/FRET

Andor SD FLIM

andor

The Andor Revolution XD is built around a Nikon Ti- inverted microscope. This system has a Yokogawa CSU-X spinning disc confocal unit with a disk speed that supports up to 2,000 frames per second. The Lambert Instruments FLIM Attachment for lifetime imaging microscopy is a dedicated system that allows rapid image acquisition and generation of lifetime images in the frequency domain. To achieve this the system uses a modulated light source and a modulated image intensifier as a detector.

FLIM WF/TIRF

LIFA WF TIRF

This system has a LIFA: Lambert Instruments Fluorescence Attachment for lifetime imaging. It is attached to a Nikon TE-2000 microscope with PFS. The LIFA system calculates lifetimes based on the frequency domain method  by using a modulated light source and a modulated image intensifier as detector. This Lambert system is coupled through our custom TIRF condenser to make it a FLIM-TIRF system for GFP.  The system can also be used for widefield FLIM.

Trimscope 1

TRIM

The LaVision TRIM microscope is a high-resolution multiphoton system able to image small groups of cells down to the level of subcellular structure and generate three-dimensional reconstructions. It is capable of fast and sectioned FLIM, as well as detecting second-harmonic generation (SHG).  The system has a heated stage and CO2 so is suitable for live-cell imaging.   

This system has been used to image neurons of the central nervous system and epithelial cells within the crypts of the colon.

Trimscope 2

The LaVision TRIM microscope is a high-resolution multiphoton system able to image small groups of cells down to the level of subcellular structure and generate three-dimensional reconstructions. It is capable of fast and sectioned FLIM, as well as detecting second-harmonic generation (SHG).

This system is capable of in vivo imaging using e.g. abdominal or mammary windows. In addition, this system has dual channel FLIM detectors so can do simultaneous FLIM measurements of CFP and GFP-based biosensors.

Specialised Techniques

Nikon TIRF 

TIRF

The facility has a total internal reflection fluorescence (TIRF) microscope built around a Nikon Eclipse inverted stand. TIRF is a powerful technique that uses an evanescent wave to excite fluorophores that are present at the bottom of the specimen adjacent to the glass water interface. The optical section that is excited is in the range of 100nm-150nm. It is often employed to study cellular membrane activities, the dynamics of actin, cellular adhesion, cell movement, single molecular events, vesicle and protein tracking. One of the advantages of TIRF is that the background and out of focus signals are dramatically reduced, which allows you to view very dim fluorescent events. This system is appropriate for live-cell fluorescent protein imaging.

PRIMO

PRIMO

The PRIMO device allows users to micropattern custom microenvironments for fixed- and live-cell experiments. The PRIMO is coupled to a fluorescent microscope and uses photopatterning technology to generate both 2D and 3D microfabricated functionalised structures.

https://www.alveolelab.com/our-products/primo-micropatterning/

Nanosight LM10

Nanosight

A Nanosight LM10 allows rapid and accurate analysis of the size distribution and concentration of all types of nanoparticles from 10nm to 2000nm in diameter, depending on the instrument configuration and sample type. The nanosight can be used to detect exosomes and microvesicles which are secreted from cells and tissues.

Leica Laser Microdissection

LMD

Laser microdissection uses a laser to isolate specific microscopic regions from tissue samples. The system is built around  a Leica DM6000B upright microscope stand.  These samples can be further analysed using additional techniques such as proteomics and metabolomics.

Zeiss Microinjection

MI

The microinjection system consists of a Zeiss Axio Observer A1 microscope equipped with an Eppendorf Femtojet and Inject man N12. This system is mainly used to inject cells with nucleic acids or therapeutics.

In Vivo Imaging

Zeiss Multiphoton 880

The Zeiss Multiphoton 880 with Airyscan is a super-resolution-capable confocal microscope. This system is in an upright configuration, and is suited to in vivo imaging of e.g. skull/brain, lung sections and abdominal organs with the use of vacuum windows.

880 MP Fred Frederic Fercoq

880 MP AmandaAmanda Mcfarlane

Trimscope 2

The LaVision TRIM microscope is a high-resolution multiphoton system able to image small groups of cells down to the level of subcellular structure and generate three-dimensional reconstructions. It is capable of fast and sectioned FLIM, as well as detecting second-harmonic generation (SHG).

This system is capable of in vivo imaging using e.g. abdominal or mammary windows. In addition, this system has dual channel FLIM detectors so can do simultaneous FLIM measurements of CFP and GFP-based biosensors.

IVIS Spectrum / Pearl / Ultrasound

Please contact Saadia Karim.

s.karim@beatson.gla.ac.uk

Other Equipment

Tissue Culture Microscopes

TC microscope

BAIR supports a number of tissue culture microscopes throughout the Beatson Institute. All these microscopes can be used to visualise fluorescent proteins in cells, and are coupled to a PC and camera.

Flow Cytometry

Please contact Tom Gilbey for information of the flow cytometry equipment and analysis software.

t.gilbey@beatson.gla.ac.uk

Histology

Please contact Colin Nixon for information of the Histology and Immunohistochemistry services and imaging.

c.nixon@beatson.gla.ac.uk 

Analysis

The BAIR Centre for Analysis and Visualisation of Experimental data is a dedicated analysis suite which gives users access to high-performance computers and both licenced and open-source software.

cave

Software includes: 

  •  Fiji
  •  Imaris
  •  Icy
  •  Harmony
  •  Zen
  •  NIS Elements
  •  Incucyte
  •  Cell Profiler
  •  Metamorph
  •  Phasefocus
  •  Flowjo

Research

Research Mahnoor 290x200

Read more about the Research Groups working at the Beatson Institute.

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Seminars

Seminars 2021

Find out more about our seminars including our Distinguished Seminar Programme.

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